Loop-mediated isothermal amplification assays for the rapid discrimination of <i>Treponema pallidum</i> lineages and subspecies.

ABSTRACT: Molecular surveillance aimed at identifying the subspecies and lineages of Treponema pallidum is important. Conventional molecular methods for genotyping are effective but are also time-consuming and costly. This study aimed to develop and evaluate a novel loop-mediated isothermal amplification (LAMP) method using quenching probes to quickly distinguish T. pallidum subsp. pallidum lineages (Nichols, SS14Ω-A, SS14Ω-B 1A, and SS14-East Asia) and T. pallidum subsp. endemicum circulating in East Asia. We developed novel LAMP assays for genotyping the tp0705 (amino acid variants of 506, 625, and 708) and tp0548 genes (a 9 bp insertion region). The LAMP assays were used to genotype 133 clinical samples (Nichols, n = 9; SS14Ω-A, n = 7

SUBMITTER: Imai K 

PROVIDER: S-EPMC12671098 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature