Ribosome-engineered <i>Lacticaseibacillus rhamnosus</i> GG with enhanced adhesion and immune activation via surface moonlighting proteins.

ABSTRACT: Commercial interest in using probiotics to enhance human health and animal production is growing. Accordingly, there is great demand for probiotic lactic acid bacteria exhibiting enhanced robustness and functionality. The present study focused on ribosome engineering (RE), a simple, economical, and safe non-genetically modified organism microbial breeding technology. Ribosome-engineered mutants of Lacticaseibacillus rhamnosus GG (LGG), one of the most widely used probiotic strains, were characterized. We found that an LGG strain with the K56N mutation within the rpsL gene (MT_K56N: K56N) expressed a variety of proteins, primarily moonlighting proteins, on the cell surface, likely due to increased translational efficiency and/or activated protein transport via ABC tr

SUBMITTER: Tsukagoshi M 

PROVIDER: S-EPMC12671100 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature