Coxsackievirus A10 blocks autophagosome-lysosome fusion to promote viral nonlytic spread and inflammatory cytokine release.

ABSTRACT: We aimed to demonstrate whether coxsackievirus A10 (CV-A10) perturbed the fusion between autophagosomes and lysosomes to cause viral nonlytic spread and inflammatory cytokine release at the blood-brain barrier (BBB), thereby contributing to neuropathological damage. CV-A10-induced autophagic secretory pathway at the cellular level was examined using western blot, immunofluorescence, and flow cytometry methods. Then, the mechanism of autophagic secretory pathway in the neuropathogenesis of CV-A10 infection was demonstrated by constructing a suckling mouse model and combining behavioral, pathological, and molecular biological methods. The expression levels of autophagic secretory pathway-related proteins, extracellular inflammatory cytokines, and viral particles were significantly increased

SUBMITTER: Wang R 

PROVIDER: S-EPMC12671134 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature