Amplification, purification, and lyophilization of mycobacteriophages for therapeutic use.

ABSTRACT: Infections with nontuberculous mycobacterium (NTM) strains are frequently nonresponsive to antibiotic regimens, even after multiple drugs are used for extensive periods of months or years. Therapy with bacteriophages specific for NTM clinical isolates shows considerable promise in compassionate-use single-patient cases but, like antibiotics, must be administered over long periods of time and typically intravenously. It is thus important that the phage preparations are highly purified and stable to avoid adverse reactions or decay of viability. Here, we describe methods for purification of mycobacteriophages including CsCl equilibrium gradient ultracentrifugation, desalting by either dialysis or column chromatography, and stabilization for storage by lyophilization. These phage preparations

SUBMITTER: Viland MD 

PROVIDER: S-EPMC12671170 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature