HLA-DM co-expression enhances MHC class II function in the magnetosome display system.

ABSTRACT: Efficient identification of antigenic peptides that bind to major histocompatibility complex class II (MHC II) molecules is crucial for vaccine and therapeutic development. However, mammalian expression systems are constrained by high costs and low scalability, particularly for stable peptide exchange. To address these challenges, we developed a bacterial magnetosome surface display system using Magnetospirillum magneticum, a magnetotactic bacterium that produces lipid bilayer-coated magnetosomes. This system enables the co-expression of MHC II and human leukocyte antigen DM (HLA-DM) on magnetosome surfaces via cohesin-dockerin interactions. This spatial co-localization significantly enhances MHC II stabilization and immobilization on magnetosomes. Moreover, co-expression with HLA-D

SUBMITTER: Tomoe R 

PROVIDER: S-EPMC12671180 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature