Multiplex detection and application of MALDI-TOF NAMS for porcine diarrheal pathogens.

ABSTRACT: Porcine diarrheal diseases caused by mixed viral and bacterial infections pose significant challenges to swine health and production. Rapid and accurate identification of key pathogens, including porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), porcine rotavirus (PoRV), swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine bocavirus (PBoV), hepatitis E virus (HEV), and Salmonella, remains a challenge due to overlapping clinical symptoms and co-infections. The zoonotic potential of HEV and PBoV also highlights the need for reliable, high-throughput detection methods. We developed a multiplex Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Nucleic Acid Mass Spectrometry (MALDI-TOF NAMS) assay for t

SUBMITTER: Shuai J 

PROVIDER: S-EPMC12671185 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature