Dynamic redox monitoring in differentiated human neuroblastoma models of Parkinson's disease.

ABSTRACT:

Background

Oxidative stress plays a critical role in the degeneration of midbrain dopaminergic (DA) neurons in Parkinson's disease (PD). However, therapies targeting redox mechanisms are hindered by a lack of scalable and inexpensive redox-focused preclinical models.

Methods

We stably expressed the glutathione-specific redox-sensitive fluorescent protein Grx-roGFP2 in SH-SY5Y and BE(2)-M17 neuroblastoma cell lines. We developed an improved differentiation protocol using staurosporine and dbcAMP to enhance dopaminergic-like characteristics, assessed by DA marker expression, and characterized responses to PD-relevant toxins.

Results

BE(2)-M17 cells expressed higher DA markers than SH-SY5Y cells, and the improved protocol further increased DA markers. Differentiated neur

SUBMITTER: Lim J 

PROVIDER: S-EPMC12671422 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature