Investigating insulin's role in regulating local aromatase in growth plate development.

ABSTRACT:

Introduction

Obesity-associated hyperinsulinemia is hypothesized to disrupt linear growth trajectories, though the mechanisms remain unclear. This study investigates these mechanisms using a high-fat diet-induced rat model.

Methods

Twelve healthy 1-week-old Sprague-Dawley rats (60-80 g) were randomized into two groups: a control group fed a standard diet and an obese group fed a high-fat diet. After 6 weeks of dietary intervention, weekly body weight and naso-anal length were recorded. At the end of the study, all rats were euthanized, and blood samples were collected for further analysis. Serum biochemical parameters, including insulin levels, were measured by ELISA. Tibiae and humeri were harvested for bone length measurement. Growth plates were isolated for histological a

SUBMITTER: Hu S 

PROVIDER: S-EPMC12671795 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature