Development and validation of a simple, cost-effective competitive allele-specific PCR assay for largescale screening and detection of FecB mutation in sheep.

ABSTRACT: The Booroola fecundity (FecB) gene, a mutation in the bone morphogenetic protein receptor-1B (BMPR1B), is known to increase ovulation rate and litter size in sheep. Efficient and accurate, large-scale detection of this single nucleotide polymorphism (SNP) is critical for marker assisted introgression/selection programs for genetic improvement of prolificacy. This study aimed to develop and validate a robust, cost-effective, and high-throughput genotyping assay for detecting the FecB mutation across diverse sheep populations globally. A competitive allele-specific PCR (KASP) assay targeting the A746G nucleotide substitution in BMPR1B gene was designed and tested on a diverse panel of 224 individuals across 22 sheep breeds and validated against direct sequencing. The KASP genotyping results

SUBMITTER: Periasamy K 

PROVIDER: S-EPMC12671827 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature