Impaired humoral and T helper cell responses to toxic shock syndrome toxin-1 (TSST-1) in granulomatosis with polyangiitis.

ABSTRACT:

Objectives

Nasal carriage of toxic shock syndrome toxin-1 (TSST-1)-positive Staphylococcus aureus (SA) is associated with a high relapse rate in granulomatosis with polyangiitis (GPA), suggesting TSST-1's role in disease progression. This study investigated the immune response to S. aureus-derived TSST-1 and characterized TSST-1-specific Th cells in GPA patients.

Methods

Plasma anti-TSST-1 IgG levels were measured in 45 remission GPA patients and 25 healthy controls (HCs) using ELISA. Circulating TSST-1-reactive CD4+T helper (Vβ2+Th) cells were analysed and sub-classified in 62 remission GPA patients and 22 HCs by flow cytometry. Additionally, PBMCs were stimulated in vitro with TSST-1 for 14 days to evaluate its effect on PR3-ANCA production and CD4+Th cell cytokine product

SUBMITTER: Liao H 

PROVIDER: S-EPMC12671868 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature