Highly different levels of natural transformation are associated with genomic subgroups within a local population of Pseudomonas stutzeri from soil.

ABSTRACT: A highly sensitive and specific PCR-based method of monitoring 16S rRNA genes of Pseudomonas stutzeri was developed for searching P. stutzeri DNA in environmental samples. This monitoring was combined with a reliable and sensitive method for isolating P. stutzeri colony formers from soil and sediment, depending on their utilization of ethylene glycol, starch, and maltose. With these techniques, P. stutzeri populations (n = 2 to 170) were obtained from five of six sites giving positive PCR signals (including three marine sediment and two soil samples). The phylogenetic positions of isolates from the five sites, based on their 16S ribosomal DNA sequences, indicated that the environmental isolates were affiliated with different genomovars of P. stutzeri. Using the broad-host-range plasmid pNS

SUBMITTER: Sikorski J 

PROVIDER: S-EPMC126724 | biostudies-literature | 2002 Feb

REPOSITORIES: biostudies-literature