Novel floxed cannabinoid receptor 2 mouse line combines knockout capability with dual fluorescent reporters.

ABSTRACT:

Background

The cannabinoid receptor 2 (CB₂) is involved in regulating immune responses, yet its specific function in microglia remains poorly defined. This study aimed to generate and validate a microglia-specific, inducible CB₂ knockout mouse model incorporating reporter genes to enable precise detection of CB₂ expression and CB₂ knockout.

Methods

A novel floxed CB₂ mouse line was generated, incorporating GFP and tdTomato reporter genes driven by the Cnr2 promoter to indicate CB₂ expression and CB₂ knockout, respectively. This line was crossed with Cx3cr1 or Tmem119 tamoxifen-inducible Cre lines to achieve macrophage- or microglia-specific CB₂ knockout, respectively. Behavioural t

SUBMITTER: Laloli KJ 

PROVIDER: S-EPMC12672438 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature