Zinc transporter ZIP10 supports zinc homeostasis and myoglobin biosynthesis in differentiating C2C12 myoblasts.

ABSTRACT:

Background

Zinc is an essential micronutrient required for diverse cellular processes, including skeletal muscle development and regeneration. Although skeletal muscle contains a large proportion of total body zinc, the mechanisms that maintain zinc homeostasis during myoblast differentiation, particularly under zinc-limited conditions, remain poorly understood.

Methods

We investigated ZIP10 (SLC39A10) as the primary zinc importer responsible for maintaining intracellular zinc homeostasis under fluctuating zinc conditions during C2C12 myoblast differentiation. Zinc levels were modulated using zinc chelation or supplementation, and ZIP10 expression was repressed by gene silencing. Molecular and functional signatures of myogenesis were assessed through gene and protein express

SUBMITTER: Shim G 

PROVIDER: S-EPMC12672441 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature