Integrating transcriptomic and proteomic analyses reveals impaired carbohydrate metabolism in tobacco cytoplasmic male sterility.

ABSTRACT: Cytoplasmic male sterility (CMS) is a vital tool for exploiting heterosis to enhance agricultural productivity. However, the genetic and molecular mechanisms of CMS in tobacco remain largely unclear. In this study, we used tobacco Gexin 3 CMS lines and their corresponding homozygous maintainer lines to systematically analyse the regulatory networks underlying CMS using transcriptomic, proteomic and morphological techniques. Morphological observations revealed premature stamen degeneration in CMS lines, resulting in failed self-pollination. Integrated transcriptomic and proteomic analyses identified 5,024 differentially expressed genes (DEGs) and 159 differentially expressed proteins (DEPs). Pathway enrichment analysis revealed that carbohydrate metabolism is a critical process in CMS. Seve

SUBMITTER: Hou C 

PROVIDER: S-EPMC12672501 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature