Yes-associated protein regulates autophagy to restore skin barrier function in atopic dermatitis.

ABSTRACT:

Introduction

Atopic dermatitis (AD) is characterized by skin barrier dysfunction and inflammation.This study explored the relationship between Yes-associated protein (YAP) expression, autophagy, and skin barrier dysfunction in AD.

Methods

Skin samples from AD patients and healthy controls were analyzed using mRNA-seq. Differential gene expression was visualized using ggplot2 and analyzed using GO and KEGG pathway analyses. An AD mouse model was created using house dust mite ointment, and HaCaT cells were treated to mimic AD inflammation. YAP expression was transfected with lentivirus in vivo and siRNA in vitro. Autophagy was induced with rapamycin.

Results

YAP levels were reduced in AD patients and correlated with filaggrin (FLG). YAP overexpression in m

SUBMITTER: Jia J 

PROVIDER: S-EPMC12672505 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature