Production of ¹³C-labeled docosahexaenoic acid from heterotrophic marine microorganisms <i>Aurantiochytrium mangrovei</i> and <i>Crypthecodinium cohnii</i> enabling fluxomic applications.

ABSTRACT: Docosahexaenoic acid (DHA, 22:6n-3) is the predominant polyunsaturated fatty acid in the human brain and eyes, playing a crucial role in vision and cognitive development. DHA deficiency has been associated with ocular diseases, such as macular degeneration and glaucoma, as well as neurodegenerative disorders. Since the human body has a limited ability to synthesize DHA from its precursor, alpha-linolenic acid (ALA, 18:3n-3), targeted DHA supplementation is essential for these patients. To investigate DHA metabolism and integration, researchers commonly use stable (²H,¹³C) or radioactive (³H,¹⁴C) isotopes, which are expensive and not widely accessible, restricting the scope and duration of studies. This study aimed to develop a sustainable method

SUBMITTER: Buscaglia M 

PROVIDER: S-EPMC12672524 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature