Multi-marker comparative analysis of 18S, ITS1, and ITS2 primers for human gut mycobiome profiling.

ABSTRACT:

Background

Gut fungi play crucial roles in human health. The profiling of the human gut mycobiome continues to progress. However, adjustments in the selection of ribosomal DNA marker regions can substantially affect the taxonomic resolution of a population. In particular, the impact of using primers' combinations is insufficiently defined. In this study, we investigated the performance of three targeted sequencing regions, ITS1, ITS2 and 18S rRNA, separately and in combination.

Methods

Eight fecal samples from healthy individuals (n = 4) and cancer patients (n = 4) were selected as proof of principle for amplicon-based sequencing conducted with the DNBSEQ™ sequencing system. Quality-filtered reads were grouped into operational taxonomic units (OTUs) via USEARCH and categoriz

SUBMITTER: Orsud H 

PROVIDER: S-EPMC12672528 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature