PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast.

ABSTRACT: SUMOylation, a conserved post-translational modification in eukaryotes, regulates protein function, localization, and stability. However, the role of SUMO chains in genome maintenance is still emerging. Using Schizosaccharomyces pombe, we show that loss of SUMO chains results in spontaneous replication stress, DNA damage, and elevated centromeric recombination. To investigate SUMO-dependent interactome at the sites of Rad52 repair, we used a split-SUMO-ID proteomics approach. It allows the analysis of local SUMOylation content at the Rad52 repair sites, and enabled the identification of the essential replication factor PCNA. We found that SUMO chain-modified PCNA antagonizes Rad8-mediated PCNA polyubiquitination, modulating the choice of post-replication repair pathways at stalled forks wi

SUBMITTER: Markowska K 

PROVIDER: S-EPMC12672585 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature