Amino acids and their metabolites as potential biochemical markers in postmortem vitreous humour.

ABSTRACT:

Background

Putrefaction is the microbial metabolism/degradation of autolysis end products like amino acids. The aim of this study was to identify and investigate amino acids and their bacterial degradation products/metabolites by LC-HRMS as possible markers of putrefaction.

Methods

Pig eyes (n = 60) were stored in conical centrifuge tubes and petri dishes within a climate chamber at 25°C, 65% humidity over four days. Each day, eyes were macroscopically examined and vitreous humour was drawn from up to six eyes. Twenty-one amino acids/metabolites (lysine, histidine, arginine, N²-acetyllysine, N²-acetylarginine, N⁶-acetyllysine, N-acetylcadaverine, tyrosine, N-acetylagmatine, tyramine, phenylalanine, N^2,6-diacetyllysine, 2-phenylethy

SUBMITTER: Franke L 

PROVIDER: S-EPMC12672791 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature