Integrated 3D microstructured digital microfluidic platform for advanced 3D cell culture.

ABSTRACT: Three-dimensional (3D) cell culture systems better simulate the in vivo microenvironment by promoting intercellular interactions and functional expression, which are crucial for tissue engineering and regenerative medicine. However, conventional two-dimensional (2D) culture platforms fail to mimic the spatial complexity of in vivo tissues, often resulting in altered cellular behavior and limited physiological relevance. In this research, we introduce a 3D cell culture platform based on a digital microfluidic (DMF) system. This platform integrates DMF electrode actuation with 3D-printed microstructure arrays, enabling precise capture and aggregation of cells within a defined 3D scaffold. While cells initially adhere in a 2D structure, they rapidly self-assemble into a 3D cell spheroid on th

SUBMITTER: Chen X 

PROVIDER: S-EPMC12673123 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature