Mechanosensitive Piezo1 Channels Mediate Diaphragm Fibrosis Induced by Prolonged Mechanical Ventilation.

ABSTRACT:

Background

Ventilator-induced diaphragmatic dysfunction (VIDD) is a major complication in critically ill patients. Prolonged mechanical ventilation (MV) triggers diaphragmatic fibrotic remodelling, but the underlying mechanisms remain unclear. This study investigated the role of the mechanosensitive channel Piezo1 in this process.

Methods

A rat model of MV was established for 6 or 12 h. Diaphragm structure (atrophy and fibrosis) and function (frequency-contraction curve and fatigue index) were assessed. The roles of Piezo1 were probed using the inhibitor GsMTx4 (a nonspecific mechanosensitive channel inhibitor) and adeno-associated virus (AAV)-mediated knockdown. Downstream signalling was identified by RNA sequencing (RNA-seq) and validated with cytosporone-B (CsnB, a specif

SUBMITTER: Xie D 

PROVIDER: S-EPMC12673280 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature