Reference Values for B Vitamins in Human Milk: The Mothers, Infants and Lactation Quality (MILQ) Study.

ABSTRACT: This third article in the series presenting reference values (RVs) for nutrients in human milk describes the values for B vitamins. The mothers, infants, and lactation quality (MILQ) and early-MILQ studies, conducted at sites in Bangladesh, Brazil, Denmark, and The Gambia, were designed to measure human milk nutrient concentrations of well-nourished mothers during the first 8.5 mo of lactation. Applying ultrahigh-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) to analyze multiple B vitamins simultaneously produced RVs for vitamin B2, B3, pantothenic acid, B6, and biotin. Choline was analyzed separately by UPLC-MS/MS, vitamin B1 by high-performance liquid chromatography-fluorescence detection, and vitamin B12 by competitive chemiluminescence enzyme immunoassay. Measured mil

SUBMITTER: Allen LH 

PROVIDER: S-EPMC12673392 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature