Noninvasive Evaluation of Trop2 Expression Using ⁶⁴Cu-NOTA-Trodelvy-F(ab')₂ in Gastric and Pancreatic Cancer.

ABSTRACT: This study aimed to develop and evaluate a ⁶⁴Cu-labeled NOTA-conjugated F(ab')₂ fragment of Trodelvy for ImmunoPET imaging of Trop2 expression in gastric and pancreatic cancer models. Trodelvy was enzymatically digested using IdeS protease to generate F(ab')₂ fragments, which were subsequently conjugated to a NOTA chelator and radiolabeled with ⁶⁴Cu. In vitro binding affinity and cellular uptake were assessed in Trop2-positive and Trop2-negative cancer cell lines using flow cytometry, immunofluorescence, and cell binding assays. In vivo ImmunoPET imaging and biodistribution studies were conducted in subcutaneous xenograft models. In NCI-N87 xenografts, ⁶⁴Cu-NOTA-Trodelvy-F(ab')₂ showed peak tumor uptake at 12 h

SUBMITTER: Huang W 

PROVIDER: S-EPMC12673595 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature