Nigericin-Triggered Phosphodynamics in Inflammasome Formation and Pyroptosis.

ABSTRACT: Innate immune signaling relies heavily on phosphorylation cascades to mount effective immune responses. Although traditional innate immune signaling cascades following TLR4 stimulation have been investigated through a temporally quantitative phosphoproteomic lens, far fewer studies have applied these methods to distinct signaling following the inflammasome trigger leading to IL-1β release. Here, we conducted time-resolved phosphoproteomic profiling to investigate kinase signaling downstream of the inflammasome trigger nigericin. We found that nigericin induces rapid and potent alterations in the phosphorylation landscape where immune-related signaling, mitogen-activated protein kinases (MAPKs), and PKC signaling are prevalent. We also found significant evidence of phospho-modified metaboli

SUBMITTER: Bhushan V 

PROVIDER: S-EPMC12673643 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature