Generation and characterization of an ovine cell line derived from peripheral blood and its potential use in the study of livestock and zoonotic viral infections.

ABSTRACT:

Background

Mesenchymal stromal cells (MSCs) are a population of undifferentiated, non-hematopoietic fibroblast-like cells isolated from several tissues with multipotent differentiation capacity in vitro. This study focused on the establishment and characterization of a mesenchymal stromal cell line derived from ovine peripheral blood mononuclear cells (PBMCs).

Methods

MSCs were isolated from ovine blood and used to establish a continuous cell line. Several assays were performed to characterize this cell line and confirm its mesenchymal origin. First, cells were characterized by flow cytometry (FACS) using monoclonal antibodies specific for MSCs and hematopoietic non-stromal cell surface antigens. Second, real-time quantitative PCR (RT-qPCR) was performed using gene primer pa

SUBMITTER: Moreno S 

PROVIDER: S-EPMC12673784 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature