Isoformic: a workflow for transcript-level RNA-seq interpretation.

ABSTRACT: Transcriptome analysis is one of the bases of modern biology, yet it is typically performed at the gene level, ignoring the complexity of alternative splicing and differential transcription initiation/termination events. Over 95% of mammalian genes produce multiple transcripts, yet most RNA-seq analyses rely on short-read data, for which transcript-level interpretation remains challenging. Current tools suffer from low accuracy, inconsistency with annotations, and lack quick solutions for downstream biological interpretation. Here, we present Isoformic, a customizable R pipeline for transcript-level analysis of short-read RNA-seq data, available on GitHub. Isoformic processes differential expression results to detect genes with transcript-level changes, visualize exon-intron structures, an

SUBMITTER: Mamede I 

PROVIDER: S-EPMC12673842 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature