Human neutrophil-derived extracellular vesicles induce renal endothelial inflammation in critical illness: an ex vivo investigation.

ABSTRACT:

Background

Circulating neutrophil-derived extracellular vesicles (NEVs) may contribute to the pathophysiology of acute kidney injury by causing glomerular endothelial inflammation.

Methods

NEVs were first isolated from ex vivo, lipopolysaccharide stimulation of whole blood taken from healthy volunteers (median age [interquartile range {IQR}]: 32 [26-42] yr; 47% female), and also from plasma of COVID-19 patients with acute respiratory distress syndrome (median age [IQR]: 59 [52-66] yr; 45% female). NEVs were incubated for 4 h in a co-culture of peripheral blood mononuclear cells and either human umbilical vein endothelial cells or renal glomerular endothelial cells. Enzyme-linked immunoassays (tumour necrosis factor-alpha [TNF]) and flow cytometry (median fluorescence intensi

SUBMITTER: Stephens JR 

PROVIDER: S-EPMC12674056 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature