Reagentless Real-Time ATP Monitoring with New DNA Aptamers.

ABSTRACT: Adenosine triphosphate (ATP) is essential to numerous biological processes, and there is considerable interest in methods for measuring this molecule in real time. Current ATP sensors have fundamental shortcomings that limit their utility, such as poor specificity, the need for exogenous reagents, or assay complexity. Aptamer-based biosensors have shown great promise for ATP detection, but existing aptamers have modest affinity and poor specificity that limit their practical utility. Here, the systematic evolution of ligands by exponential enrichment (SELEX) process is used to discover new DNA aptamers that bind ATP with unprecedentedly high affinity and specificity. The best-performance aptamer, ATP18-13, displays at least tenfold greater affinity for ATP relative to previously reported a

SUBMITTER: Bacon A 

PROVIDER: S-EPMC12674096 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature