Automated Microfluidic Platform for Single Spheroid Culture and Extracellular Vesicle Isolation: Application to Spheroid Transcriptomic Profiling.

ABSTRACT: Extracellular vesicles (EVs) are key mediators of intercellular communication and carry molecular information that reflects the state of their cell of origin. 3D cell cultures more accurately reflect the in vivo microenvironment and the biogenesis of extracellular vesicles compared to 2D cultures. Despite these advantages, studying EVs in 3D systems such as spheroids remains technically challenging. Conventional EV isolation and characterization methods often require pooling multiple spheroids to obtain sufficient material, which masks the intrinsic heterogeneity between individual spheroids and limits applications in precision medicine. To overcome these challenges, this work develops an automated microfluidic platform capable of single-spheroid culture, continuous secretion collection, a

SUBMITTER: Hut M 

PROVIDER: S-EPMC12674101 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature