Mapping the proteome landscape of Indian Zebu (Sahiwal) spermatozoa using high-resolution mass spectrometry and <i>in-silico</i> annotation.

ABSTRACT: Proteomic analysis of sperm cells offers significant insights into proteins' structural, functional, and localization aspects within biological systems. Sahiwal, a native Indian cattle breed, is well known for its disease resistance, calving ease, and resilience to drought. This study addressed the gap in Sahiwal's comprehensive sperm proteome profiling data. The research involved the global in-silico quantitative high-resolution mass spectrometry-based protein profiling of Indian Zebu sperm, identifying 4651 sperm proteins. Beyond mere identification, the study characterized these proteins at a sub-organellar level to facilitate a better understanding of their functional attributes. Gene Ontology analysis of sperm proteins facilitated the segregation of proteins based on their function, l

SUBMITTER: Bhushan V 

PROVIDER: S-EPMC12674427 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature