Translocation of benzo(a)pyrene reactive metabolites across human mammary epithelial cell membranes.

ABSTRACT: DNA adducts from benzo(a)pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAH) are related to tumor initiation in many tissues including mammary epithelia. T47D mammary cells are able metabolizers of BaP, forming DNA and cellular protein adducts but also a sizeable amount of extracellular protein adducts. GSH S-transferases (GST) help mitigate adduct formation by glutathione (GSH) conjugation. Here, we varied GSH levels using buthionine sulfoximine (BSO) and BaP pretreatments to deplete or augment GSH, respectively, to study adduct formation and metabolism at 4 µM 3H-BaP over 24-48hr. An inverse relationship was observed between GSH levels and nuclear protein and DNA adducts. Time course experiments showed extracellular protein adducts, identified primarily as bovine serum albumin

SUBMITTER: Merrick BA 

PROVIDER: S-EPMC12674524 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature