Assessing NaV1.7 during tonic firing in pig C-nociceptors.

ABSTRACT: Assuming the voltage-gated sodium channel (VGSC) NaV1.7 facilitates action potential generation upon slow electrical depolarization, we investigated protoxin II and TTX to target VGSC sub-types and to assess their role in C-fiber excitability when stimulated with sinusoidal single 1 Hz pulse (500 ms) and repetitive 4 Hz stimuli. We performed ex vivo extracellular compound potentials (CAP) recordings of pig saphenous nerve and in vivo pig single nerve fiber (SNF) recordings of heat- mechanosensitive ("polymodal") nociceptors (C-HT) and low- threshold mechanoresponsive C-fibers (C-LTMR) upon electrical 1 and 4 Hz sinusoidal stimulation, which evoke a discharge burst and a tonic response, respectively. Both toxins reduced C-CAP amplitudes and conduction velocity. Number of action potentials e

SUBMITTER: Soares S 

PROVIDER: S-EPMC12674544 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature