Single-Cell RNA Sequencing Reveals Peritoneal Environment and New Insights into Fibrosis in a Continuous Ambulatory Peritoneal Dialysis Patient: A Longitudinal Self-Controlled Study from Dialysis Initiation to 3-Year Follow-Up.

ABSTRACT:

Introduction

Peritoneal fibrosis (PF) from long-term peritoneal dialysis (PD) is one of the main reasons for the ultrafiltration failure and the abandonment of PD in patients, and there is no effective treatment available. The dynamic changes in the PD microenvironment and their relationship to PD-related fibrosis remain unclear.

Methods

We performed single-cell RNA sequencing (scRNA-seq) on overnight PD effluent collected from a continuous ambulatory PD (CAPD) patient at two critical time points: the initiation of dialysis and after 3 years of treatment, so as to reveal the dynamic changes of immune cells and peritoneal mesothelial cells (PMCs) during prolonged PD.

Results

The results showed that six distinct populations of cells were identified within the PD effluen

SUBMITTER: Zheng S 

PROVIDER: S-EPMC12674672 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature