In vitro dynamic and quantitative monitoring of strigolactone-signaling complex formation by time-resolved FRET.

ABSTRACT: Strigolactones (SLs) are a class of plant hormones that play a critical role in the suppression of shoot branching. Furthermore, they are exuded from roots and act as signaling molecules for inter-organism communication in the rhizosphere. Strigolactones trigger those responses by inducing protein-protein interactions (PPIs) of signaling components and subsequent proteolysis of transcriptional repressors. The sequential event involves SL hydrolysis mediated by SL receptors belonging to an α/β-hydrolase family, although the physiological role of SL hydrolysis is a subject of debate. To date, SL-induced PPIs have been analyzed by methods such as yeast-two hybrid, pull-down, and AlphaScreen assays. However, the kinetic aspect of PPI profiles has not been well studied. Here, we developed an in

SUBMITTER: Suzuki T 

PROVIDER: S-EPMC12674854 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature