A novel mouse cell line model reveals the tumor intrinsic and immune characteristics of EGFR-mutant lung cancer.

ABSTRACT: Background: Epidermal growth factor receptor (EGFR) mutations occur frequently in lung adenocarcinoma (LUAD). However, the lack of practical preclinical models has limited our understanding of how EGFR mutations reshape the tumor immune microenvironment. Methods: EGFR^{E19del/E19del}; Trp53^-/- (EP) cell lines were established from a human EGFR exon 19 deletion mutated genetically engineered mouse model (GEMM). These cell lines were applied to generate orthotopic tumors in both immunodeficient and immunocompetent mice. Drug sensitivity assays were performed to evaluate responses to Osimertinib. Multi-omics analyses, including transcriptomic and metabolic profiling, were conducted to compare EP tumors with KRAS-mutant models and human EGFR-mutant LUAD. Res

SUBMITTER: Yan Y 

PROVIDER: S-EPMC12675003 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature