Spatial metabolic gradients in the liver and small intestine.

ABSTRACT: The properties of mammalian cells depend on their location within organs. Gene expression in the liver varies between periportal and pericentral hepatocytes^1-3, and in the intestine from crypts to villus tips^4,5. A key element of tissue spatial organization is probably metabolic, but direct assessments of spatial metabolism remain limited. Here we map spatial metabolic gradients in the mouse liver and intestine. We develop an integrated experimental-computational workflow using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS), isotope tracing and deep-learning artificial intelligence. Most measured metabolites (>90%) showed significant spatial concentration gradients in the liver lobules and intestinal villi. In the liver, tricarboxy

SUBMITTER: Samarah LZ 

PROVIDER: S-EPMC12675281 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature