Mitochondrial damage and IL-1β production in monocytes caused by <i>Neospora caninum</i> infection are mediated by dense granule protein 7 and prohibitins.

ABSTRACT:

Introduction

The intracellular proliferation of Neospora caninum tachyzoites and the host immune response against infection are key steps in the pathogenesis of neosporosis. However, the molecules responsible for the activation of the inflammasome induced by N. caninum infection have not been identified.

Methods

In the infection of human monocytic cell line THP-1 cells with the N. caninum dense granule protein 7 knock-out (NcGRA7KO) parasite and the parental strain Nc1, production of IL-1β and TNF-α, phosphorylated NF-κB p65 were measured. LC‒MS/MS analysis of NcGRA7 immunoprecipitates, mitochondrial fractionation and proteolysis assays were also performed.

Results

In the infection of THP-1 cells with the NcGRA7KO parasite, decreased IL-1β release

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12675473 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature