Nuclear effects play an important role in determining codon usage-dependent human gene expression.

ABSTRACT: Codon usage biases play a significant role in determining gene expression levels. Codon usage was thought to primarily influence translation-dependent processes. Using an unbiased genome-wide screening to search for factors involved in codon usage effects on gene expression, we identified the CCR4-NOT complex subunit CNOT4 and many nuclear factors, including nuclear RNA exosome, PAXT complex components, and transcription factors. The CCR4-NOT complex has been shown to affect codon usage-dependent co-translational mRNA decay in yeast. Surprisingly, human CNOT4 was found to influence codon usage-dependent gene expression largely by its impact on nuclear RNA levels due to transcriptional effect. On the other hand, nuclear exosome and PAXT complex, regulate nuclear mRNA stability through the R

SUBMITTER: Garg R 

PROVIDER: S-EPMC12675662 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature