MiR-495-3p Attenuates Neuronal Apoptosis Through The Activation of Wnt/MAPK Pathways in Ischemic Stroke.

ABSTRACT: The incidence of ischemic stroke, involving neuronal cell death and damaged blood vessels, is gradually increasing. In this study, we investigated the effects of a miR-495-3p-I on neuronal viability and recovery using both oxygen-glucose deprivation and recovery (OGD/R) and in transient middle cerebral artery occlusion (tMCAo) models of ischemic stroke. Cell viability increased with miR-495-3p-I treatment compared with the OGD/R group, and both early and late apoptosis decreased through by flow cytometry analysis. In the tMCAo model, tissues injected with miR-495-3p-I showed upregulation of canonical Wnt pathway genes (cyclin D1 and c-Myc) and neuronal marker (Tuj1), while non-canonical Wnt/MAPK pathway genes (caspase-3 and -7) were downregulated. Expression of the pro-apoptotic marker Bax

SUBMITTER: Jang E 

PROVIDER: S-EPMC12675737 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature