Isolation and partial characterization of antimicrobial peptide-enriched fractions from Parabacteroides distasonis.

ABSTRACT: Parabacteroides distasonis is a part of the indigenous human microbiota and plays a significant role in disease etiopathogenesis. This study aimed to evaluate the efficacy of peptides isolated from P. distasonis strains. We focused on the expression, purification, characterization, antimicrobial efficacy through antagonism assays, and in silico studies of antimicrobial peptides produced by 78 P. distasonis strains. Methodology: Seventy-eight P. distasonis strains isolated from broiler (Gallus gallus domesticus) feces were evaluated. Antagonistic activity was assessed using the double-layer diffusion method. The influence of pH, temperature, proteolytic enzymes, and organic solvents on the intracellular peptide fraction (C-50) was analyzed. MIC was determined according to CLSI guidelines wi

SUBMITTER: Oliveira AGG 

PROVIDER: S-EPMC12675784 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature