Neuroprotective Proteins in Hypoxia-stressed Astrocyte-Derived Extracellular Vesicles.

ABSTRACT:

Background

Advances in mass spectrometry-based proteomic analysis have generated extensive protein data from cells involved in neurodegenerative diseases. The field of neuroproteomics is expanding to include the study of extracellular vesicles (EVs) to identify potential biomarkers for disease prevention and endogenous factors involved in neuroprotection.

Methods

In this study, rat cortical astrocytes in normoxia were cultured under normoxic conditions and subsequently exposed to hypoxia. Astrocyte-derived EVs released into the supernatant were collected separately from both conditions. Label-free mass spectrometry-based proteomics was then performed to assess the effects of hypoxia on the EV protein cargo. A meta-analysis comparing the results with previously published EV p

SUBMITTER: Bernal-Vicente BN 

PROVIDER: S-EPMC12676041 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature