Separation and detection of Gram-negative bacteria via vancomycin-functionalized magnetic beads and aminopeptidase test strips.

ABSTRACT:

Introduction

Gram-negative bacteria are key pathogens causing food contamination and animal diseases (such as bovine mastitis), and they are prone to developing multidrug resistance, necessitating rapid and sensitive detection technologies.

Methods

We developed a novel detection method based on an antibiotic affinity strategy, which combines vancomycin-functionalized magnetic beads (Van-MBs) with aminopeptidase test strips. Vancomycin, which binds exclusively to D-alanyl-D-alanine (D-Ala-D-Ala) on the cell wall of Gram-positive bacteria, is used by Van-MBs to capture Gram-positive bacteria from the sample. After magnetic separation, the supernatant containing Gram-negative bacteria is retained. This supernatant reacts with aminopeptidase test strips, utilizing the enzyme's u

SUBMITTER: Xu X 

PROVIDER: S-EPMC12676118 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature