The SIMPLIFY Protocol: A Monophasic Extraction System Suitable for Exposomics, Metabolomics, Lipidomics, and Proteomics Research.

ABSTRACT: Advancing our understanding of human health and disease requires comprehensive analytical approaches capable of capturing the complex interplay between endogenous metabolism and environmental exposures. A major challenge in clinical research is the ability to capture multidimensional data, particularly a broad range of biochemical profiles, due to limitations of biological resources, time, and budget. In this study, we introduce the SIMPLIFY Protocol, a unified monophasic extraction method that enables the simultaneous extraction of chemical exogenous products and endogenous molecules. The method was evaluated against in-house extraction techniques, including protein precipitation with methanol (MeOH) and acetonitrile (ACN), and the Folch method using various sample types, particularly cer

SUBMITTER: Nguyen AH 

PROVIDER: S-EPMC12676512 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature