Comparative Analysis of Sample Loop and Counting Bead-Based Methods for Size-Dependent Bias in Flow Cytometry.

ABSTRACT: Reliable and accurate particle number concentration measurements are essential across various fields, including clinical diagnostics, environmental monitoring, and industrial applications. Flow cytometry is widely used for these measurements, where the use of counting beads is a common approach. However, this method can introduce size-dependent bias when the target particles differ in size from the counting beads. To evaluate size-dependent bias, this study systematically compares the conventional counting bead-based method with a sample loop-based method that relies on total counting with a defined sample volume. Experimental results show that while both methods yield similar concentrations for beads of comparable size, discrepancies arise when there are significant size differences betwe

SUBMITTER: Shin HJ 

PROVIDER: S-EPMC12676513 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature