Cx43 hemichannel-mediated paracrine involves in the promotion of MSCs on skin wound healing.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) exhibit therapeutic potential in wound healing, primarily through their paracrine effects. However, the specific mechanisms underlying these effects remain ambiguous. Connexin 43 (Cx43), the most abundantly expressed connexins in MSCs, forms hemichannels that mediate the paracrine release of small molecules, suggesting a potential role in MSC-mediated wound healing.

Methods

A full-thickness skin wound model was established in mice to investigate the role of Cx43 hemichannels in MSC-mediated wound healing. Wounds were treated with MSC-conditioned medium (MSC CM), Cx43 hemichannel-inhibited medium (18α-GA CM), or control medium (CTRL CM, complete medium). The assessment of wound healing was conducted through histological and immunofluor

SUBMITTER: Dou X 

PROVIDER: S-EPMC12676879 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature