Metagenomic analysis of microbiome spatial dynamics in urban river confluence affected by city wastewater.

ABSTRACT:

Background

Environmental pollutants have a profound impact on microbial dynamics. This study highlights the influence of anthropogenic activity on the shift in bacterial diversity in the catchment area compared to upstream and downstream at Kathajodi, using a metagenomic approach for the first time in River Kathajodi.

Methods

Water samples were collected from upstream, catchment, and downstream locations and transported at 4°C to the laboratory for DNA extraction, library preparation, sequencing, and physicochemical analysis employing inductively coupled plasma. The extracted DNA was sequenced via the Illumina HiSeq platform and analyzed through MG-RAST for taxonomic and functional classification using KEGG and COG annotations. Statistical diversity analysis, including raref

SUBMITTER: Parwin N 

PROVIDER: S-EPMC12676890 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature