Sialoglycans modulate Siglec-5-TLR4 interactions in osteoarthritis.

ABSTRACT: Osteoarthritis (OA) is characterized by chronic, low-grade inflammation that contributes to cartilage degradation and joint pain. We previously identified sialic acid-binding immunoglobulin-like lectin-5/14 (Siglec-5/14) in synovial fluid of OA patients (OA SF), which prompted us to investigate its interaction with the sialylated proinflammatory receptor Toll-like receptor 4 (TLR4) in monocytes. Here, we reveal an inverse correlation between Siglec-5 and TLR4, suggesting that Siglec-5 may suppress inflammation. To gain mechanistic insights, monocytes stimulated with OA SFs were compared to macrophage colony stimulating factor (M-CSF), lipopolysaccharide (LPS), and sialidase, to assess patient-specific inflammatory pathways and phenotypes. Notably, OA SF that triggered elevated interleukin

SUBMITTER: Raberg L 

PROVIDER: S-EPMC12677102 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature