Suppressing Glycerol-3-phosphate Phosphatase and Enhancing Glycerol-3-Phosphate Shuttle Flux Crucial for High-Efficiency Fatty Acid Production in the Fast-Growing Oleaginous Schizochytrium.

ABSTRACT: The industrial microbial production of fatty acids is limited by low titers and productivities, highlighting the need for chassis engineering and mechanistic insights into efficient metabolic pathways. The heterotrophic microalga Schizochytrium offers distinct advantages, and Schizochytrium ZW1 is engineered into a high-efficiency fatty acid production chassis. A fluorescence-activated cell sorting strategy is developed to overcome cellular aggregation, enabling the rapid isolation of Schizochytrium ZW2, which exhibited enhanced growth and fatty acid accumulation. Multiomics analysis of ZW2 revealed that mutations suppressing glycerol-3-phosphate phosphatase activity promoted triacylglycerol synthesis, and mutations in the polyunsaturated fatty acid synthase C subunit increased polyunsatur

SUBMITTER: Wang F 

PROVIDER: S-EPMC12677618 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature