BLOC1S1 Attenuates B. Melitensis 16M LPS-Triggered Autophagy by Spatial Confinement of TDP-43.

ABSTRACT: Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC1S1) is considered to have anti-Brucella potential. However, the effect of BLOC1S1 on Brucella autophagy has not yet been studied. This study investigates the interplay between Brucella lipopolysaccharide (LPS) and BLOC1S1 in modulating autophagy within goat spermatogonial stem cells (mGSCs-I-SB). Using LPS from B. melitensis 16M, its capacity is demonstrated to induce AMPK-dependent autophagy, contrasting with Escherichia coli LPS, which shows no significant effect. Mechanistically, B. melitensis 16M LPS activates AMPK signaling, elevates LC3B-II/LC3B-I ratios, and upregulates lysosomal and pro-inflammatory genes. BLOC1S1 overexpression attenuates autophagy, reducing autolysosome formation (TEM) and LC3B-II/I ratio. RNA se

SUBMITTER: Wan S 

PROVIDER: S-EPMC12677691 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature