Design and Evaluation of Sticky End-Type Bivalent DNA Aptamers Containing M08s-1 as Anticoagulant Agents.

ABSTRACT: Aptamer is a promising therapeutic modality, offering strong target affinity and target versatility. In the previous studies, DNA aptamers with potent thrombin inhibitory activity, M08s-1 and its bivalent derivatives, have been developed as novel anticoagulant agents. Among them, the heterobivalent aptamer Pse08-29 exhibits the most potent ex vivo anticoagulant activity, surpassing the approved drug argatroban. However, its long oligonucleotide chains composed of 106 nucleotides may hinder its drug development due to limited chemical synthetic efficiency. Here, a segmented form of Pse08-29 composed of two DNA strands, assembled via a sticky end-type strategy, is developed. The cleavage positions and optimal linker lengths are studied based on the anticoagulant activity and structural stabi

SUBMITTER: Yokomori M 

PROVIDER: S-EPMC12677840 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature